![]() ![]() tuberculosis, 40% of the individuals that become infected will develop primary active tuberculosis, and 60% remain with the latent form of the bacilli and may present extrapulmonary sites of infection, resulting from inefficient macrophage action at the beginning of exposure. Mycobacterium tuberculosis causes pulmonary tuberculosis (Tb), and the clinical manifestations of infection can be either acute, or latent and asymptomatic, depending on the intensity of the immune response mounted by the infected patient.Īfter being exposed to M. tuberculosis recombinant antigens diagnosis. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient. Western blot specificity was 100% when antibody reactivity with different antigenic bands was analyzed and associated. ELISA sensitivity improved from 85% to 92% when the Western blot results were used. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity and 91% specificity. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. Phone: (55) 220 recombinant antigens and a crude bacterial antigen of a wild M. IVBiolab-Merieux S/A and Tropical Medicine Institute, São Paulo, SP, Brazilĭepartamento de Análises Clínicas e Toxicológicas IIClinical Hospital Faculty of Medicine of São Paulo University, São Paulo IFederal University of Santa Maria, Santa Maria, RS tuberculosis recombinant antigens diagnosisĬombined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis You will find general information about cookies and details on how to delete cookies from your device.Two recombinant antigens and a crude bacterial antigen of a wild M. Alternatively, you can visit which contains comprehensive information on how to do this on a wide variety of browsers and devices. If you want to restrict or block the cookies that are set by our website, you can do so through your browser setting. For example, we will recognize your username and remember how you customized the site during future visits. Functionality cookiesįunctionality cookies let us operate the site in accordance with the choices you make. For example, these cookies let us recognize that you have created an account and have logged into that account. Necessary cookies allow us to offer you the best possible experience when accessing and navigating through our website and using its features. What types of cookies do we use? Necessary cookies It will contain some anonymous information such as a unique identifier, website’s domain name, and some digits and numbers. Each cookie is unique to your web browser. What are cookies?Ĭookies are simple text files that are stored on your computer or mobile device by a website’s server. By visiting plati.market you agree that plati.market may use cookies to be processed by Google Analytics and Yandex.Metrika. Please read this cookie policy carefully before using plati.market operated by us. ![]()
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